Abstract: The alignment of artificial intelligence values has distinct advantages in exploring how human subjectivity can be better protected. It is an important attempt to continuously gain mutual trust between humans and artificial intelligence, and sets a good example for the development of emerging technologies represented by artificial intelligence towards goodness. From the perspective of the beneficial dimension of subjectivity in aligning the value of artificial intelligence, it is highlighted in three aspects: enriching the connotation of human subjectivity, ensuring the integrity of human subjectivity, and highlighting the status of human subjectivity. However, it cannot be ignored that the value alignment of artificial intelligence poses a potential threat to human freedom and comprehensive development, mainly manifested as the “masking” of human emotions, cognition, and activities, which poses a risk of damaging human subjectivity. By enhancing emotional interaction, promoting algorithm transparency, and strengthening practical participation, we can construct a way out to uncover subjectivity and better ensure that human subjectivity can play a greater role on a healthy track. Human subjectivity is the endogenous driving force behind the progress of artificial intelligence, and the alignment of the value of artificial intelligence provides a guarantee for better stimulating the internal vitality of human subjectivity, promoting the better sublimation of human technological civilization.